Increasing TB diagnosis through use of Truenat technology

Increasing TB diagnosis through use of Truenat technology

Truenat is a molecular diagnostic equipment developed by Molbio Diagnostics, Goa, India. It uses portable, battery operated devices to rapidly detect Mycobacterium tuberculosis complex bacteria (MTBC) and rifampicin resistance. The system involves two main devices;

The Trueprep™ AUTO Sample Prep Device and the Truelab™ Real Time micro PCR Analyzer. Truenat is seen as a promising new TB diagnostic tool since it’s more sensitive and specific than microscopy, has minimal infrastructure requirements and can be used as near-POC and results are rapidly available allowing for same-day diagnosis and can detect RIF resistance within two hours.
With funding from USAID, SRL Uganda has recently installed 38 Truenat devices to support in diagnosis of TB and drug resistant TB across the county. This is to further increase access to early, rapid and quality TB diagnosis through use of WHO recommended molecular diagnostic tools.

Figure 1: National Truenat TOT at NTRL-Butabika

Figure 2: Training on Truenat in Toroma HCIV-Katakwi district

 

District Health facility
Katakwi District Toroma HC IV
Terego District Omugo HC IV
Yumbe District Midigo HC IV
Yumbe District Yumbe HC IV
Amudat Karita HCIV
Rukiga District Kamwezi HC IV
Kasese District Rukoki HC IV
Buliisa District Biiso HCIV
Kabarole District Bukuku HC IV
Kakumiro Kakumiro HCIV
Kabarole District Rutete HCIV
Kisoro District Rubuguri HC IV
Tororo District Mukuju HC IV
Tororo District Nagongera HC IV
Manafwa District Bugobero HC IV
Mbale District Bufumbo HC IV
Mbale District Namatala HC IV
Sironko District Buwasa HC IV
Butaleja District Nabiganda HC IV
Nakaseke District Ngoma HCIV
Lwengo District Kiwangala HC IV
Kalangala District Bukasa HC IV
Kalaki District Lwala hospital
Sheema Shuuku HCIV
Ibanda District Ishongororo HC IV
Isingiro District Rwekubo HC IV
Kotido Kanawat HC III
Kamuli District Kagumba HC IV
Bugiri District Nankoma HC IV
Kamuli District Namwendwa HC IV
Lira District Amach HC IV
Amuru District Kaladima HCIII
Wakiso District Kabubbu HCIV
Wakiso District Bweyogerere HCIIII
Nakaseke District Semuto HC IV
Kampala National TB Reference Laboratory
Luwero District Nyimbwa HC IV
Wakiso District Buwambo HC IV

 

 

Use of TB-LAMP as a replacement for microscopy
Loop mediated Isothermal amplification (LAMP) is a unique temperature independent technique for amplifying DNA that was developed by Eiken Chemical Company (Totyo, Japan). It is simple to use, providing a visual display. Additionally, the technique is robust and can be used at peripheral health facilities where microscopy is performed. TB-LAMP is a manual assay that requires 1 hour to perform and results can be read by naked eye under UV light. TB LAMP requires minimal infrastructure and Biosafety requirements that is similar to sputum smear microscopy. Uganda is implementing TB-LAMP in 16 health facilities across the country to increase capacity for TB diagnosis. It is also envisaged that due to the high throughput of TB-LAMP (maximum capacity of 70 samples per day), this tool will be important in supporting TB testing in seasons of TB campaign which generate high volumes of samples.

Figure1: TB LAMP equipment set up at Namalu HCIII, Nakapiripirit District.

Figure 2: Junior Manager-Human Diagnostic Worldwide and CEO Human Diagnostic Ugand Ltd handing over of Humaloop-T equipment to Director of SRL

 

Table 2: List of TB-LAMP facilities

Jinja Jinja Municipal HCIII
Mbale Nakaloke HCIII
Moroto Nadugent HCIV
Nakapiripiriti Namalu HCIII
Napak Lopei HCIII
Nabiratuk Lolachat HCIII
Soroti Princess Diana HCIV
Arua St. Assumpta HCIII
Gulu Layibi Techo HCIII
Lira P.A.G HCIV
Hoima Azur HCIV
Kasese Kasese Municipal HCIV
Mbarara Mbarara Municipal HCIV
Kabale Rushoroza HCIV
Masaka Kitovu Hospital

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